Peptide Ligand Variants Elicit Differential oGPCR Signaling Responses, Related to Figure 5
(A) BB3 responses for neuromedin B (NMB) and gastrin-releasing peptide (GRP) cleavage variants.
(B) GPR1 responses for gastrin-releasing peptide, osteocrin (OSTN) and cholecystokinin (CCK-33) cleavage variants.
(C) GPR55 responses for PACAP cleavage variants. Mass redistribution data show an apparently biphasic response for PACAP peptides, which could indicate an additional intracellular signaling pathway mediated via GPR55. Numbers in parenthesis indicate positions in the full-length protein.All data represent mean ± SEM for n = 3-4 independent experiments performed in triplicate.