Figure S7.

Confirmation of Proposed Pairings and Secondary Ligands for Known Peptide Receptors, Related to Figure 7

(A) Of the 14 pairings proposed in literature, half were reproduced in mass redistribution and/or internalization assays (Table S5). Note: P2RY10–lysophosphatidylserine (LPS) activity was marginal. ^∗We tested cleavage variants of cortistatin (CST-14) and somatostatin (SRIF-28).

(B-C), Nine known peptide receptors were activated by their cognate agonists (internal controls) and, unexpectedly, 22 additional peptides. These indicate as yet unappreciated cross-pharmacology (Table S7). All data represent mean ± SEM for n = 3 independent experiments performed in triplicate.