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. 2019 Oct 17;179(3):671–686.e17. doi: 10.1016/j.cell.2019.09.022

Figure S1.

Figure S1

Nups Form Compositionally Diverse Granules, Related to Figure 2

(A) Classes of Nup granules. (A-A’’) Top view confocal images of a fixed GFP::Nup358 expressing egg chamber stained for WGA-Alexa647, labeling FG-Nups. GFP::Nup358 and WGA colocalize at the NE of nurse and follicle cells and at AL in the ooplasm (A). In addition, GFP::Nup358 localizes to bright spherical Nup358 granules that are prevalent in nurse cells (yellow arrowheads in A). In the ooplasm, WGA stains dual labeled AL and oocyte specific granules that do not contain GFP::Nup358.

(B and C) Granules are not due to overexpression of Nup107. Top view confocal images of fixed egg chambers where RFP::Nup107 rescues the null allele nup107E8. In the absence of endogenous Nup107, RFP::Nup107 condenses into oocyte specific granules and localizes to AL (B-B’’). RFP::Nup107 condensation in nurse cells is rare (C-C’’) and might be confined into Nup358 granules as exemplified in Figure 4A and 4C.