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. 2019 Nov 1;10:2462. doi: 10.3389/fimmu.2019.02462

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Copyright © 2019 Hawez, Al-Haidari, Madhi, Rahman and Thorlacius.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mimic miR-155 upregulates and AntagomiR-155 downregulates PAD4 mRNA expression in neutrophils stimulated with PMA (500 nM). (A) miR-155-5p and (B) PAD4 mRNA expression in cells transfected with Ctrl Mimic and mir-155 mimic. (C) miR-155-5p and (D) PAD4 mRNA expression in cells transfected with Ctrl-antagomiR and antagomiR-155-5p. Relative expression was determined by qRT-PCR. U6 and GAPDH were used as a house-keeping genes to normalize miR-155-5p and PAD4 expression, respectively. Relative expression was determined using 2^−ΔΔCT method. Data represent mean ± SEM and n = 5. ^#P < 0.05 vs. control and *P < 0.05 vs. vehicle.