Figure 2.

663-optOva Has Superior Capacity to Generate an Ag-Specific Immune Response

(A) Analysis of IFNγ secretion by spleen CD4⁺ T cells stimulated with MHC class II Ova-immunodominant peptide Ova_323–339 2 weeks after immunization measured by ELISPOT assay. (B) Levels of Ova_257–264- tet⁺ CD8⁺ T cells in the blood 2 weeks after vaccination. (C) The representative analysis of changes in activation markers on cell surface of Ova_257–264- tet⁺ CD8⁺ T cells compared to naive cells in the blood 3 weeks after vaccination. OD, optical density. (D) Levels of terminally activated and memory precursor effector T cells after vaccination, which were calculated after analysis of data. Cell populations were defined as follows: T effector (TE) cells: Ova_257–264- tet⁺/CD44⁺/CD62L⁻; terminally differentiated T effector (terminal TE): Ova_257–264- tet⁺/CD44⁺/CD62L⁻/KLRG1⁺/CD127^−; T effector memory (TEM): Ova_257–264- tet⁺/CD44⁺/CD62L⁻/KLRG1⁻/CD127⁺; and T central memory (TCM): Ova_257–264-tet⁺/CD44⁺/CD62L⁺/KLRG1⁻/CD127⁺. ∗ p < 0.05.