Figure 3.

Comparison of Functional Activity of Ova-Specific CD8⁺ T Cells after 663-Ova and 663-optOva Vaccination

(A–C) Functional analysis 4 weeks after vaccination. (A) IFNγ production by splenocytes re-stimulated with Ova_257–264 peptide (SIINFEKL) 4 weeks after immunization. (B) In vivo cytotoxicity assay 4 weeks after immunization. Syngeneic naive splenocytes were divided into two equal parts and labeled with low and high concentrations of CFSE. The fraction labeled with low CFSE concentration was also pulsed with Ova_257–264 peptide before splenocytes were mixed and adoptively transferred to immunized mice. 16 h later, CFSE-labeled populations were analyzed in the spleen. (C) Representative results for each group. (D–F) Functional analysis 9 weeks after vaccination. (D) IFNγ production by splenocytes re-stimulated with Ova_257–264 peptide (SIINFEKL) 9 weeks after immunization. (E and F) In vivo killing capacity of mice immunized 9 weeks before analysis. 16 h after adoptive transfer of labeled splenocytes. *p < 0.05; **p < 0.01.