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. 2019 Aug 16;18:617–626. doi: 10.1016/j.omtn.2019.08.012

Figure 3.

Figure 3

PRKCB Was Upregulated in GC and Positively Related to hsa_circ_0092306

(A) Quantitative real-time PCR showed PRKCB was significantly upregulated in 45 paired GC and adjacent tissues. (B) The PRKCB expression was positively related to the hsa_circ_0092306 expression in 45 GC tissues. (C) The expressions of PKCβ1 protein and PRKCB mRNA in the normal gastric mucosal epithelial cells and GC cells were detected by western blot and quantitative real-time PCR. (D and E) The expressions of PKCβ1 protein and PRKCB mRNA after regulating the hsa_circ_0092306 expression were detected in MKN-45 cells (D) and tumor tissues (E). (F) The expression of PKCβ1 protein after regulating the hsa_circ_0092306 expression was determined by immunohistochemistry. **p < 0.01 and ***p < 0.001 meant statistical significance.