Figure 4.

miR-197-3p Targeted hsa_circ_0092306 and PRKCB

(A) Quantitative real-time PCR was performed and showed a lower level of miR-197-3p in 45 paired GC and adjacent tissues. (B) There was a negative relationship between hsa_circ_0092306 and miR-197-3p (R² = 0.3678). (C) hsa_circ_0092306 suppressed the miR-197-3p expression in MKN-45 cells. (D) miR-197-3p was upregulated in the hsa_circ_0092306 knockdown tumors. (E) There was a negative relationship between PRKCB and miR-197-3p (R² = 0.6766). (F) The targeting sequence between hsa_circ_0092306 and miR-197-3p was revealed by the Circular RNA Interactome, and the luciferase reporter experiment indicated the target relationship of hsa_circ_0092306 and miR-197-3p. (G) miR-197-3p enriched in the pull-down production with the hsa_circ_0092306 probe. (H) The targeting sequence between PRKCB and miR-197-3p was revealed by the TargetScan, and the luciferase reporter experiment indicated the target relationship of PRKCB and miR-197-3p. **p < 0.01 and ***p < 0.001 meant statistical significance.