Fig. 2.

Metformin ameliorates PM_2.5-induced pulmonary fibrosis and inflammation. (A) Representative lung sections from the PBS- and PM_2.5-exposed WT and AMPKα2^−/− mice with or without metformin (Met) treatment were stained with hematoxylin and eosin (H&E), Masson's trichrome, and antibodies specific for neutrophils and macrophages (galectin-3, Gal-3) (brown staining). Scale bar = 100 μm. The relative collagenous fiber area (B), and the number of Gal-3 positive cells (C) and neutrophils (D) were quantified. N = 4; ** indicates p < 0.01. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)