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. 2019 Aug 5;27(11):1906–1918. doi: 10.1016/j.ymthe.2019.07.019

Figure 4.

Figure 4

CD8+ T Cells Mediate the Antitumor Activity of Ad5-PC

Male C57BL/6 mice were injected peritoneally with 5 × 106 H22 cells. Mice were treated i.p. with recombinant adenoviruses (5 × 108 PFUs/mouse), combined with or without anti-CD8a or anti-NK1.1. (A) Schematic diagram of the experimental setup for CD8+ T cell or NK cell depletion. (B) Mouse blood was collected 5 days after the administration of blocking antibody, and the depletion effects of anti-CD8a and anti-NK1.1 were confirmed via flow cytometry. (C) Survival curves of mice treated with recombinant adenoviruses in the presence or absence of anti-CD8a or anti-NK1.1 antibodies. The concentrations of soluble proteins (D) and IFN-γ (F) in ascites were determined by ELISA. (E) Viral copies in ascites were detected by qPCR. Data shown are the means ± SD. #Not significant; *p < 0.05, ***p < 0.001.