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The effects of melatonin on infarct size. Melatonin (0.3 and 50 μM) administration was given for 10 min immediately before and for 10 min during reperfusion after 35min coronary artery ligation, followed by 60 min reperfusion. Tetraphenyltetrazolium staining was used to assess infarct size which was expressed as a % of the area at risk (A,B).
