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. 2019 Nov 1;5(10):e02659. doi: 10.1016/j.heliyon.2019.e02659

Fig. 2.

Fig. 2

Fig. 2

Mitochondrial oxidative phosphorylation function after exposure of the hearts to ischaemia and reperfusion: effects of melatonin. Melatonin (0.3 and 50 μM) was administered to isolated perfused hearts for 10 min before and for 10 min after ischaemia and mitochondria isolated for subsequent evaluation of mitochondrial oxidative phosphorylation function after (i) stabilization for 30 min (ii) stabilization followed by 20 min global ischaemia and (iii) stabilization, followed by 20 min global ischaemia and 30 min reperfusion. Respiratory activities were measured in the presence of glutamate (5mM) plus malate (2mM) (substrates for complexes I and II) or palmitoyl-L-carnitine (0.45mM) plus malate (2 mM) (mitochondrial fatty acid beta-oxidation substrate). Parameters evaluated were ADP/O ratio (Supplementary file Fig. 1); QO2 (State 3) (nAtoms oxygen uptake/mg protein/min in presence of ADP) (A); QO2 (State 4) (nAtoms oxygen uptake/mg protein/min after phosphorylation of added ADP) (B); oxidative phosphorylation rate (nmoles ATP produced/mg protein/min) (Supplementary file Fig. 2). Abbreviations: Stb: stabilization; Isch: 20 min global ischaemia; Rep: reperfusion after 20 min global ischaemia. n = 4–6 hearts/group.