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. 2019 Nov 1;5(10):e02659. doi: 10.1016/j.heliyon.2019.e02659

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© 2019 Published by Elsevier Ltd.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 4 — The effect of melatonin on indicators of mitochondrial mitophagy in hearts subjected to ischaemia and reperfusion. Hearts were subjected to 20 min global ischaemia followed by 30 min reperfusion with and without melatonin (0.3 μM) (Fig. 2) for subsequent preparation of mitochondria. Immunoblots and summary of the effects of 0.3 μM melatonin on p62 (A), TOM70 (B), PINK1 (C) and Parkin (D) expression in mitochondria isolated after Stb, isch and rep. For full non-adjusted images of gels see Supplementary file1 Figs 4A-D. Abbreviations: see Fig. 3. P62: p62/SQSTM1; TOM70: translocase of outer membrane 70; PINK1: PTEN-inducible kinase 1.

Fig. 4 — The effect of melatonin on indicators of mitochondrial mitophagy in hearts subjected to ischaemia and reperfusion. Hearts were subjected to 20 min global ischaemia followed by 30 min reperfusion with and without melatonin (0.3 μM) (Fig. 2) for subsequent preparation of mitochondria. Immunoblots and summary of the effects of 0.3 μM melatonin on p62 (A), TOM70 (B), PINK1 (C) and Parkin (D) expression in mitochondria isolated after Stb, isch and rep. For full non-adjusted images of gels see Supplementary file1 Figs 4A-D. Abbreviations: see Fig. 3. P62: p62/SQSTM1; TOM70: translocase of outer membrane 70; PINK1: PTEN-inducible kinase 1.