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Soft-agar assay. Evaluation of MDA-MB-231 colony formation after 18 days of incubation with 5% of pre- or post-exercise human sera. s-01, subject 01; s-02, subject 02; s-03, subject 03. Data are expressed as mean ± SEM of four experiments; **p < 0.01, ***p < 0.001, respect to t0.
