Figure 6.
Systematic Toxicity Evaluation of Ld0-GFP in BALB/c Mice
(A) Treatment scheme. i.v., intravenous. BALB/c mice were injected with KOS, d0-GFP, and Ld0-GFP viruses at the indicated dosage and followed for survival. (B) Body weight monitoring of BALB/c mice after receiving a single dose of 5 × 107 PFU viruses through i.v. injection. Scale bars, 100 μm. (C) H&E staining of representative tissue sections from vehicle-, KOS-, d0-GFP-, and Ld0-GFP-injected mice on days 1, 5, 7, and 30 following virus injection. Black arrows represent lung injuries. The statistical significance of the intergroup comparisons of body weight was analyzed using a repeated-measure ANOVA. All values are presented as the mean ± SEM. ***p < 0.001.