Figure 2.

Model parameter estimation. (A) Reduction of image resolution by pixel binning. Reported value of resolution is res = 1/n (where n × n pixels have been averaged and condensed into a single pixel; res = 1 is the experimentally acquired image). (B) Measured CVs at division (symbols) at four different image resolutions (as in A) and therefore with four different experimental errors σ_ε. Lines show fitting of these CV data by tuning the parameters of the model. See also Fig. S2. Dashed line is located where σ_ε = Rσ_ρ: for smaller errors, area sensing becomes apparent, i.e., ${\text{CV}}_{A_d^{*}}<{\text{CV}}_{L_d^{*}}$. WT strain FC15, n = 326. (C) Measurement of division asymmetry and its fitting with a Gaussian distribution to determine σ_α (n = 106), where L^∗_s-t is the measured distance between the division septum and cell tip. Experimental data are fitted with σ_α^∗ = 0.035. Because ${\sigma }_{\alpha \ast }^2\approx {\sigma }_{\alpha }^2+\left(5{\sigma }_{\epsilon }^2/L^2\right)$, we can extract σ_α = 0.032.