Figure 1.

Schematic diagram of the integrated holographic optical tweezers (HOT) and Raman microscope. The instrument allows cyclical stretching of individual GUVs using three laser beams; the same laser beams are used to excite Raman measurements. For a vesicle, the elongation factor e is defined as the ratio between the long and short semiaxes. DCM, dichroic mirror; DMD, digital micromirror device; DRC, camera imaging the DMD; IOM, inverted optical microscope; IS, imaging spectrometer; MSC, microscope side-port camera; NF, notch filter; OBJ, microscope objective; SLM, spatial light modulator; TEL, telescope. To see this figure in color, go online.