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Spatially resolved Raman spectra of typical Chol/POPC/SM GUVs. (a) Relaxed vesicle and (b and c) two elongated vesicles stretched using the HOT are shown. The bright-field images (DMD camera) of the trapped GUVs, both in the relaxed and stretched state, show the positions of the laser beams for stretching and measuring Raman spectra. Scale bars, 5 μm. To see this figure in color, go online.
