Figure 3.

(a) Raman spectra measured at the edges of a typical Chol/POCP/SM GUV cyclically stretched using HOT. Spectra are normalized using the intensity of the d⁺ band (2847 cm⁻¹). (b) Calculated intensity ratios I₂₈₄₇/I₂₈₈₂ and I₂₈₄₇/I₂₉₂₅ at different elongation factors for the GUV in (a) are shown. (c) Intensity ratios I₂₈₄₇/I₂₈₈₂ and I₂₈₄₇/I₂₉₂₅ calculated from Raman spectra of additional seven GUVs are shown. Each guiding line corresponds to individual GUVs. Horizontal error bars represent uncertainties in identifying the coordinates of the GUV edges (the vertical error bars are negligible). To see this figure in color, go online.