Figure 5.
(a) Spatially resolved Raman spectra for a typical Chol-d6/POPC/SM GUV at different elongation factors showing the C-D bands. Spectra are vertically offset for clarity. (b) Fit of the ratio of the C-D band intensities measured at the center and edge of a vesicle ICD(center)/ICD(edge) to the theoretical model (Eqs. 1, 2, and 3) is shown for two GUVs (data points indicated by black squares and white circles). (c) Fit of the band intensity ratio ICD(center)/ICD(edge) to the theoretical model for data obtained from 17 GUVs is shown, yielding a mean value r = 0.63. The lines corresponding to the ±0.05 error range (r = 0.68 and r = 0.58) are also included. Error bars indicate estimated experimental uncertainties in determining e. To see this figure in color, go online.