Table 2.

The broth^‡ acidification rate by novel LAB strains isolated from silage growing in wells with 48-h old biofilms of pathogenic bacteria

	Glucose	Strains	L. plantarum AG 1	P. acidilactici AG 8	L. plantarum AG 9	L. plantarum AG 10	L. plantarum AG 15	L. fermentum AG 16	L. plantarum 8PA3
ΔpH	2%	LAB monoculture	3.4 ± 0.35*	3.2 ± 0.24*	3.4 ± 0.31*	3.6 ± 0.56*	3.3 ± 0.23*	3.2 ± 0.11*	2.3 ± 0.15
		P. aeruginosa	2.5 ± 0.55	2.6 ± 0.22*	2.5 ± 0.54	2.5 ± 0.5	2.4 ± 0.39	3.1 ± 0.91*	2.4 ± 0.51
		S. aureus	3.1 ± 0.21*	3.1 ± 0.14*	3.1 ± 0.21*	3.1 ± 0.23*	3.1 ± 0.23*	3.2 ± 0.25*	2.8 ± 0.25
		E. coli	2.0 ± 0.11	2.0 ± 1.08	2.0 ± 1.10	2.0 ± 1.15	2.0 ± 1.15	1.8 ± 1.44	1.8 ± 1.11
		B. cereus	3.3 ± 0.18*	3.4 ± 0.23*	3.2 ± 0.25*	3.1 ± 0.06*	3.1 ± 0.09	3.4 ± 0.50*	2.9 ± 0.41
	0.2%	LAB monoculture	2.5 ± 0.34*	2.0 ± 0.53*	2.2 ± 0.46*	1.8 ± 0.27*	2.2 ± 0.38*	2.2 ± 0.62*	1.2 ± 0.25
		P. aeruginosa	0.8 ± 0.79	1.0 ± 0.76*	0.2 ± 0.38	0.1 ± 0.88	1.0 ± 0.86*	1.0 ± 0.88*	0.7 ± 0.43
		S. aureus	1.7 ± 0.64*	1.6 ± 0.47*	1.7 ± 0.52*	1.7 ± 0.66*	1.7 ± 0.64*	1.6 ± 0.54*	0.6 ± 0.30
		E. coli	0.5 ± 0.68	0.1 ± 0.50	0.4 ± 0.37	0.5 ± 0.64*	0.6 ± 0.80*	0.3 ± 0.56	0.2 ± 0.62
		B. cereus	0	0	0	0	0	0	0
ΔTTA	2%	LAB monoculture	2.2 ± 0.63*	1.5 ± 0.43*	1.7 ± 0.65*	2.3 ± 0.87*	1.8 ± 0.57*	1.3 ± 0.23*	1.1 ± 0.01
		P. aeruginosa	0.3 ± 0.12	0.3 ± 0.10	0.3 ± 0.09	0.3 ± 0.05	0.3 ± 0.07	0.6 ± 0.03*	0.3 ± 0.04
		S. aureus	1.1 ± 0.21*	1.3 ± 0.18*	1.5 ± 0.65*	1.1 ± 0.21*	1.1 ± 0.21*	1.3 ± 0.34*	0.5 ± 0.02
		E. coli	0.7 ± 0.20	0.5 ± 0.15	0.5 ± 0.08	0.6 ± 0.09	0.6 ± 0.06	0.6 ± 0.06	0.6 ± 0.01
		B. cereus	1.3 ± 0.25*	1.3 ± 0.31*	1.5 ± 0.74*	1.3 ± 0.21*	1.4 ± 0.21*	1.4 ± 0.76*	0.7 ± 0.03
	0.2%	LAB monoculture	0.31 ± 0.11	0.30 ± 0.10	0.31 ± 0.08	0.30 ± 0.05	0.27 ± 0.03	0.25 ± 0.05	0.31 ± 0.06
		P. aeruginosa	0.03 ± 0.01	0.03 ± 0.01	0.05 ± 0.02	0.03 ± 0.01	0.02 ± 0.01	0.03 ± 0.02	0.01 ± 0.02
		S. aureus	0.05 ± 0.01	0.01 ± 0.01	0.05 ± 0.01	0.02 ± 0.02	0.05 ± 0.03	0.05 ± 0.02	0.02 ± 0.01
		E. coli	0.08 ± 0.01	0.50 ± 0.01	0.52 ± 0.01	0.5 ± 0.02	0.07 ± 0.02	0.23 ± 0.02	0.28 ± 0.09
		B. cereus	0.02 ± 0.01	0.05 ± 0.01	0.05 ± 0.01	0.03 ± 0.01	0.05 ± 0.02	0.05 ± 0.01	0.01 ± 0.02

^‡ Suspensions of LAB (10⁷ CFU/mL) in MRS broth without sorbic acid were added into the wells with 48-h old biofilms pre-formed by pathogenic bacteria and incubation was continued for the next 24 h. ΔpH and ΔTTA were calculated as a difference between initial and final values. The Total Titratable Acidity (TTA) is expressed as mL of 0.1 M NaOH as required to achieve the final pH of 8.2

* denotes statistically significant difference with probiotic strain L. plantarum 8PA3 (p < 0.05)