Fig. 3.

PMCA2 levels in the lumbar DH of male C57Bl/6NTac mice with EAE manifesting pain. Evaluation of thermal (heat) and mechanical pain sensitivity in male mice at early phase of EAE by a the Hargreaves test and b von Frey filament test. c–e PMCA2, PMAC3, and PMCA4 protein levels in the lumbar DH. The graphs (upper panel) show the quantification of the band intensity in the Western blots (lower panel; two representative lanes per group). Total protein was used to normalize for experimental variations. The number of mice in each group is shown above bars. Values represent mean ± SEM. Significantly different by independent Student’s t test with ***p < 0.001 and ****p < 0.0001