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. 2019 Nov 8;16:207. doi: 10.1186/s12974-019-1585-2

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — PMCA2 levels in the lumbar DH of mice sustaining a SCI and manifesting chronic pain. Assessment of the a Hargreaves test and b von Frey filament test in mice sustaining a SCI at 28 dpi. c PMCA2 transcript levels in the lumbar DH of mice at 28 dpi. d PMCA2, e PMAC3, and f PMCA4 protein levels in the lumbar DH of mice at 28 dpi. Graphs (upper panels) show the quantification of the band intensity in the Western blots (lower panels; two representative lanes per group). Total protein was used to normalize for experimental variations. The number of mice in each group is shown above bars. Values represent mean ± SEM. Significantly different by one-way ANOVA and Tukey’s post hoc test. *p < 0.05, ***p < 0.001, and ****p < 0.0001