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The mechanisms of internal priming and template switching. (a) Internal priming occurs due to the annealing of a primer to an A-rich region. A-rich regions are typically defined as genomic loci with six or more consecutive As or 12 As out of 20 nucleotides. (b) Template-switching artifacts are produced when the polymerase dislocates during elongation and reinitiates at a homologous sequence of another template
