Fig. 5.

LC/MS peptide mapping of Pfs230D1+. a Reduced (top) and non-reduced (bottom) chymotrypsin-digested Pfs230D1+ base-peak ion chromatograms. b Sequence coverage of reduced or non-reduced chymotrypsin-digested Pfs230D1+ was 91% and 84%, respectively. c In the chymotrypsin-digested Pfs230DI+ base-peak ion chromatogram, peaks at ~ 57.8 min and ~ 63.8 min correspond to the N-terminal peptide with or without an additional Arg residue (+ 156 Da) on the N-terminus, respectively. The relative ion abundance of the N-terminal peptide containing the N-terminal Arg residue was 19 ± 2%, which was determined from six experiments (three independent non-reduced or reduced chymotrypsin digestions)