Fig. 4.

Optimal visualization window for HS-169 with multiphoton microscopy in the in vivo mouse brain. a. Top panel, experimental procedure to characterize HS-169 in the mouse brain in vivo. APP:PS1 Tg mice were retro-orbitally injected with HS-169 and longitudinally imaged with multiphoton microscopy for 6 weeks. Bottom panel, pictures show different time points after injection of HS-169 (red). t = 0 represents imaging a few minutes after injection. Dextran Fluorescein 70,000 Da MW (green) was systematically injected to create a fluorescent angiogram. Scale bar represents 100 μm and applies to all pictures. b. Quantitative analysis of HS-164 fluorescence intensity. Normalized to t = 24 h. Optimal imaging time was determined at 24 h after HS-169 injection in the APP:PS1 Tg mouse. n = 96 plaques from 2 mice. c. Quantitative analysis of fluorescence intensity HS-169 in the blood circulation. Normalized to t = 24 h. n = 75 vessels from mice