Fig. 5.

Co-injection of HS-84 and HS-169 help discriminating Aβ plaque subtypes in vivo in the APP:PS1 mouse living brain. a. Experimental procedure to characterize HS-169 and HS-84 in the mouse brain in vivo. APP:PS1 Tg mouse carrying a cranial window was simultaneously injected with HS-169 and HS-84 and subjected to multiphoton microscopy 24 h later. b. Representative in vivo multiphoton microscopy images of HS-84 (green) and HS-169 (red) in the same brain area in an APP:PS1 Tg mouse. Note heterogeneity among plaques. c. Examples of different amyloid beta plaques among the mouse brain. Immunohistochemistry with anti-abeta antibody (6E10) was carried out, followed by co-staining with HS-84 and HS-169 0.005% (w/v). Representative images from n = 5 mice. d. Ex-vivo co-staining of APP:PS1 Tg mouse tissue with HS-84 and HS-169. Note that within the same field of view, heterogeneity of amyloid β plaque can be observed