Fig. 1.

a RNAscope staining of IL8 (green)-CXCR2 (red) and CCL2 (red)-CCR5 (green) mRNA in a NF-PitNET and a somatotropinoma. CCL2 and IL-8 are mainly expressed in pituitary tumour cells, while the chemokine receptors are strongly expressed in scattered perivascular cells (morphologically distinct from tumour cells), likely corresponding to immune cells. Scale bar 20 μm. b Cytokine secretome from NF-PitNETs (n = 16) and somatotropinomas (n = 8). Data are shown for the top 12 secreted proteins as mean ± standard error of the mean. *, <0.05 (Mann Whitney U test). c Percentage of NF-PitNETs (n = 16) and somatotropinomas (n = 8) with detectable cytokine levels, i.e. concentration above the lowest standard curve and serum-free medium quantification. Data are shown as percentage and for the top 12 secreted proteins as identified by the Millipore MILLIPLEX assay in primary culture supernatants. *, < 0.05, **, < 0.01 (Chi-squared test)