Figure 2.

Sub-population distributions and population mapping visualize treatment-dependent spatial heterogeneity within FaDu xenografts and organoids. Population density modeling of single cell measurements of NAD(P)H τ_m in FaDu (A) xenografts and (B) organoids reveal heterogeneous cell populations within control, cetuximab-treated, cisplatin-treated, or cetuximab and cisplatin-treated (combination) groups. Representative population maps of control and treated (C) xenografts and (D) organoids demonstrate spatial organization of cell populations with differing treatment response. Individual cells are color-coded based on the population assignment determined from density-based clustering analysis. Responsive populations, corresponding to low NAD(P)H τ_m, are coded red, and resistant populations, corresponding to high NAD(P)H τ_m, are coded blue. Cell outlines are in white. Scale bar, 50 μm.