Fig. 3.

Clock-associated genes expression upon B-AZ treatment. (A) Expression of clock-associated genes in seedlings treated with B-AZ for 3 h (mean � SEM, n = 3). Asterisks and double asterisks indicate significant differences between solvent DMSO and B-AZ-treated samples (Student’s t-test P < 0.05 and 0.01, respectively). Time indicates hours after transfer to continuous light. White and gray areas were subject day and night, respectively. (B–D) PRR fusion proteins in seedlings treated with B-AZ were analyzed by Western blotting (lower). Coomassie brilliant blue (CBB) staining indicates similar amounts of total proteins were analyzed (upper). Similar results were obtained in other experiments for (A–D).