Skip to main content
. 2019 Oct 29;25:8095–8104. doi: 10.12659/MSM.916075

Figure 3.

Figure 3

XIST interacted with miR-144-3p. (A) The binding sites between XIST and miR-144-3p were predicted using the miRCode database. (B, C) The luciferase reporter assay was used to detect luciferase activity of H460/DDP cells and A549/DDP cells co-transfected with XIST wild-type (WT) or XIST mutated (MUT) and miR-143-3p mimic or negative control (NC) mimic. (D, E) The expressions of miR-143-3p and XIST were detected in A549/DDP and H460/DDP cells after Ago2 or IgG RIP assay. (F) RNA pull-down experiment was performed to determine the relationship between XIST and miR-143-3p. (G) The expression of miR-144-3p in A549/DDP and H460/DDP cells with different treatment was detected by quantitative reverse transcription polymerase chain reaction (RT-qPCR). (H) The expression of miR-144-3p was performed using RT-qPCR in normal tissues, tumor tissue, and tumor/DDP tissues. (I) The relationship between XIST and miR-144-3p were determined in tumor tissue by Pearson’s correlation analysis. * p<0.05.