Fig. 1.

Performance increase of ST holder compared to DT holder is marginal. Representative examples of specimen shift throughout a FISE dose-symmetric 3° increment tilt series recorded using a single-tilt (ST) axis holder (A/B) or a dual-tilt (DT) axis holder (C/D). The tilt axis in both cases is approximately parallel to the x-axis. Overall, specimen shifts are comparable between both holders and x-shift is negligible compared to y-shift. The DT holder shows larger systematic shift upon reversal of tilt direction (arrowhead).