Table 1.
K3 camera eliminates long processing times. Time measurements of FISE −60° to +60° tilt series with 3° increments on Titan Krios [DT/K2] and Titan Krios [ST/K3].
| Instrument | Tilt scheme | Unblank timea | Prep timeb | Set Rec timec | Cam Rec timed | Total timee |
|---|---|---|---|---|---|---|
| [ST/K3] | unidirectional | 1 s | 98 s | 91 s | 95 s | 201 s |
| [DT/K2] | unidirectional | 1 s | 136 s | 117 s | 253 s | 399 s |
| [ST/K3] | dose-symmetric | 1 s | 101 s | 130 s | 134 s | 243 s |
| [DT/K2] | dose-symmetric | 1 s | 134 s | 185 s | 339 s | 484 s |
a
Time the sample is exposed per tilt.
b
Time taken to refine eucentricity and autofocus.
c
Exposure time set for Record in SerialEM. Variations are due to the slower tilt speed on Titan Krios [DT/K2]. In all cases, the default tilt speed of the stage was not altered.
d
Time from start of acquisition until regain of microscope control.
e
Total run time of the tilt series including resetting the microscope state.