Figure 6.

Dp114RhoGEF Mediates Gβ13F/Gγ1 Signaling at Junctions

(A) Confocal acquisitions of Myo-II::mCherry in the ventro-lateral ectoderm of control, Gβ13F/Gγ1++, and Gβ13F/Gγ1++, Dp114RhoGEF shRNA embryos. The increase of Myo-II at vertical junctions observed in Gβ13F/Gγ1++ embryos is lost when Dp114RhoGEF shRNA is also expressed in the background.

(B) Mean junctional intensity of Myo-II::mCherry according to the angle of the junctions in control, Gβ13F/Gγ1++ embryos, and Gβ13F/Gγ1++, Dp114RhoGEF shRNA embryos.

(C) Amplitude of polarity of junctional Myo-II::mCherry in control, Gβ13F/Gγ1++ embryos, and Gβ13F/Gγ1++, Dp114RhoGEF shRNA embryos. Although Myo-II planar polarity increases upon Gβ13F/Gγ1 overexpression compared to control embryos, co-expression of Gβ13F/Gγ1 together with Dp114RhoGEF shRNA reduces Myo-II planar polarity, similar to Dp114RhoGEF shRNA embryos alone.

Scale bars represent 5 μm. Means ± SEM are shown. Statistical significance has been calculated using Mann-Whitney U test. ns, p > 0.05; ^∗p < 0.05; ^∗∗p < 0.01.

See also Figure S7.