Figure 3.
Effects of bromelain on clonogenic formation of AGS cells were evaluated by a clonogenic assay, using IC50: 65 μg/ml. Clonogenic assay was conducted 14 days after treatment. (a-d) Untreated cells, (e-h) treated cells with IC50 of 65 μg/ml as a function of seeded cell number per well; (a and e) 200; (b and f) 100; (c and g) 50; and (d and h) 10. (i) The percentage of plating efficiency was plotted as the number of seeded cells per well which were treated with/without bromelain. Data are presented as mean ± standard error of at least three independent experiments