Figure 5.

MCF7 cell response to bromelain after 14 days of treatment (IC₅₀ = 65 μg/ml) on clonogenic formation evaluated by a clonogenic assay. (a-d) Untreated cells and (e-h) treated cells. The function of seeded cell number per well is as follows: (a and e): 200; (b and f): 100; (c and g): 50; and (d and h) 10. (i) The percentage of plating efficiency was plotted as the number of seeded cells per well which were treated with/without bromelain. Data are presented as mean ± standard error of at least three independent experiments