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Characterization of the polyclonal antibody used to detect DENND1A.V2. (A) IF detection of DENND1A.V2 in normal theca cells. (B) Absence of signal when antibody was neutralized with immunogenic peptide. (C) IF detection of DENND1A.V2 in PCOS theca cells. (D) Absence of signal when antibody was neutralized with immunogenic peptide. Scale bars, 10 μm. Representative images from three different experiments using different cell lines are shown. 3D, three-dimensional.
