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. 2019 Sep 16;3(12):2204–2223. doi: 10.1210/js.2019-00169

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Copyright © 2019 Endocrine Society

This article has been published under the terms of the Creative Commons Attribution Non-Commercial, No-Derivatives License (CC BY-NC-ND; https://creativecommons.org/licenses/by-nc-nd/4.0/).

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Figure 3. — DENND1A.V2 colocalizes with the endosome protein RAB5B in normal and PCOS theca cells. (A) Normal untreated theca cells. (B) Normal theca cells treated with 20 μM forskolin. (C) Untreated PCOS theca cells. (D) PCOS theca cells treated with 20 μM forskolin. Scale bars, 10 μm. (E) Representative image taken using a SIM microscope, showing colocalization at higher magnification. (F) Graph showing percentage colocalization between DENND1A.V2 and RAB5B. (G) Quantification of nucleus/cytoplasm ratio for DENND1A.V2 expression. Bars with a letter are significantly different from unlettered bars and bars with a different letter (two-way ANOVA and a Newman–Keuls post hoc test). ^aStatistically significant difference between PCOS theca cells (P) vs normal theca cells (N) without forskolin treatment (P < 0.05). ^bStatistically significant difference between 0 μM vs 20 μM forskolin treatment (P < 0.05). (H) Quantification of nucleus/cytoplasm ratio for RAB5B expression. ^aStatistically significant difference between P vs N without forskolin treatment (P < 0.05). ^bStatistically significant difference between 0 μM vs 20 μM forskolin treatment (P < 0.05). Representative images from three different experiments using different theca cell preparations are shown. Data are presented as means ± SE.