Figure 3.

[Reprinted with permission from Bergholt et al. (2016a)]. Raman spectroscopic imaging of articular cartilage. (A) Univariate Raman spectroscopy images of articular cartilage showing the band intensity associated with H₂O (3,400 cm⁻¹), GAG (1,410 cm⁻¹), collagen (1,245 cm⁻¹), cytoplasm (1,345 cm⁻¹), DNA (795 cm⁻¹), apatite (960 cm⁻¹), and the overlay. Scale bar: 50 μm. (B) High-resolution (~0.3 μm) Raman spectroscopy image of chondrocytes and pericellular matrix obtained by imaging the GAG (1,410 cm⁻¹), cytoplasm (1,345 cm⁻¹), and DNA (795 cm⁻¹) against collagen (1,245 cm⁻¹). Scale bar: 3 μm. (C) Representative Raman spectra measured from articular cartilage with marked signatures for specific tissue components.