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. 2019 Nov 1;7:303. doi: 10.3389/fbioe.2019.00303

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Copyright © 2019 Bergholt, Serio and Albro.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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[Reprinted and modified with permission from Albro et al. (2018)] (A) Raw spectra of engineered cartilage tissue from chondrocyte-seeded agarose hydrogel scaffolds. Raman spectroscopic image (univariate analysis) of ECM heterogeneities in 56-day cultured large engineered cartilage tissue construct (Ã 6 × 3 mm) for (B) the full cross-section (10 μm spatial resolution) and (C) a localized peripheral region (1 μm resolution). Arrows represent media-exposed surfaces.