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. 2019 Nov 1;7:303. doi: 10.3389/fbioe.2019.00303

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Copyright © 2019 Bergholt, Serio and Albro.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Reprinted and modified with permission from Bergholt et al. (2017). Biochemically measured GAG and collagen contents of TE constructs after 0, 14, 28, 42, and 56 days of culture. (A) Mean Raman spectra ± 1 standard deviation (SD) of native cartilage and TE constructs after varying culture durations. (B,C) Correlations between the Raman prediction (in arbitrary units) and biochemically measured GAG and collagen, respectively, with linear fits to the data. Raman spectroscopy showed an excellent correlation with biochemical assays for measurement of collagen (R² = 0.84) and GAG (R² = 0.86).