Table 1.
Tentative assignments of Raman peaks of the extracellular matrix and cells.
| Raman peak | Tentative assignment | Associated molecule | References |
|---|---|---|---|
| 785–795 | νs(O-P-O) | DNA | Kuhar et al., 2018 |
| 820–939 | ν(C-C) | Proteins (collagen) | Janko et al., 2010 |
| 960 | ν(PO4) | Hydroxyapatite | Bergholt et al., 2016a |
| 1,002–1,004 | νs(C–C) | Phenylalanine of proteins | Lim et al., 2011 |
| 1,063 | νs(S = O) | GAG | Demers et al., 2015 |
| 1,080 | ν(C-C) | Lipids | Czamara et al., 2015 |
| 1,230–1,280 | Amide III | Proteins | Rygula et al., 2013 |
| 1,375–1,410 | νs(COO−) | GAGs | Bergholt et al., 2016a |
| 1,400–1,500 | b(CH2/CH3), sc(CH2/CH3), b(CH2) | Lipids | Kuhar et al., 2018 |
| 1,445–1,456 | b(CH2/CH3), sc(CH2/CH3), b(CH2) | Proteins | Lim et al., 2011; Esmonde-White, 2014 |
| 1,650–1,680 | Amide I υ(C = C) | Proteins | Rygula et al., 2013 |
| 1,745 | υ(C = O) | Lipids | Movasaghi et al., 2007 |
| 2,850–2,854 | υs(CH2) | Lipids | Czamara et al., 2015; Lu et al., 2015 |
| 2,880 | υas(CH3) | Lipids | Mo et al., 2009; Czamara et al., 2015 |
| 2,931–2,940 | υ(CH3) | Proteins | Mo et al., 2009 |
| 3,100–3,600 | υ(OH) | H2O | Leikin et al., 1997; Lu et al., 2015 |
The Raman spectra of various tissue biomolecules are highly overlapping, and the peak position is often reported with variability of ~±1–5 cm−1 due to instrument variability.