Figure 4.

Oral gavage of simvastatin and HFD educate radiation‐shifted intestinal bacterial structure at day 7 after TAI based on the gender of animal. For box plot, the top and bottom boundaries of each box indicate the 75th and 25th quartile values, respectively, and lines within each box represent the 50th quartile (median) values. Ends of whiskers mark the lowest and highest diversity values in each instance. A,B) The observed species number A) and Chao1 diversity index B) of intestinal bacteria in male mice was examined by 16S rRNA high‐throughput sequencing after 7 d of TAI exposure. Significant differences are indicated: Wilcoxon rank sum test. n = 5 (control group) or 6 (irradiated groups). C,D) The observed species number C) and Chao1 diversity index D) of intestinal bacteria in female mice was examined by 16S rRNA high‐throughput sequencing after 7 d of TAI exposure. Significant differences are indicated: Wilcoxon rank sum test. n = 5 (control group) or 6 (irradiated groups). E,F) Principle coordinate analysis (PCoA) were performed to assess the shift in intestinal bacterial composition profile from male E) and female F) mice after irradiation at day 7. n = 5 (control group) or 6 (irradiated groups). G,H) The β diversity of intestinal bacteria was compared by weighted unifrac analysis. Significant differences are indicated: Wilcoxon rank sum test. n = 5 (control group) or 6 (irradiated groups). I–L) The abundances of most varied strain bacteria in male I, J) and female K,L) mice was assessed using 16S high‐throughput sequencing after irradiation at day 7. Significant differences are indicated: Wilcoxon rank sum test. n = 5 (control group) or 6 (irradiated groups).