Skip to main content
. 2019 Jan 30;68(11):2044–2056. doi: 10.1136/gutjnl-2018-316091

Figure 3.

Figure 3

Characterisation of human bone marrow mesenchymal stem cell (hBMSC)-derived multiple immune cell lineages in hBMSC-Fah-/- Rag2-/- IL-2Rγc-/- SCID (FRGS) mice. (A) Total number of hCD45+ cells in the peripheral blood, spleen, liver, bone marrow (BM), thymus and lymph nodes in hBMSC-FRGS mice (blue circle) and control FRGS mice (black circle) at week 12 after transplantation (n=8/group). (B) Detection of human cytokine expression in hCD45+ cells isolated from the livers of hBMSC-FRGS mice and subjected to in vitro stimulation with phytohemagglutinin (PHA) (green column) or phorbol 12-myristate 13-acetate (PMA)/ionomycin (violet column) for 24 hours (n=6/group). hCD45+ cells without stimulation (black column) were used as controls. (C) Detection of hBMSC-derived human immune cells in the livers of hBMSC-FRGS mice at 12 weeks after transplantation by immunohistochemistry (IHC) for B cells (hCD19+), T cells (hCD4+ and hCD8+), natural killer (NK) cells (hNKp46+), macrophages (hCD86+ and hCD163+) and dendritic cells (DCs) (hCD11c+ and hCD123+) (bar=100 µm). (D) Gating scheme and representative fluorescent-activated cell sorting (FACS) contour plots of hBMSC-derived multiple human immune cell lineages in the livers of hBMSC-FRGS mice. (E) Reconstitution and maintenance of hCD45+ cells, hCD19+ B cells, hCD3+ T cells and hCD3-hNKp46+ NK cells in the peripheral blood, spleen and livers of hBMSC-FRGS mice from week 3 to 60 after transplantation (10 different donors, n=3). Relative proportions of (F) hCD4+ helper T (TH) cells and hCD8+ cytotoxic T (TC) cells within the population of hCD45+hCD3+ T cells in the spleen and liver of hBMSC-FRGS mice (n=6/group); (G) hCD86+ M1 and hCD163+ M2 subset within the population of hCD45+hCD3-hCD14+hCD68+ macrophages in the spleen and liver of hBMSC-FRGS mice (n=6/group) and (H) hCD11c+ myeloid and hCD123+ plasmacytoid subsets within the population of hCD45+hCD3-hCD14-HLA-DR+ DCs in the spleen and liver of hBMSC-FRGS mice (n=6/group). a) P<0.05; b) p<0.01; c) p<0.001). U.D., undetectable.