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. 2019 Nov 8;14(11):e0225081. doi: 10.1371/journal.pone.0225081

Fig 5. Subcellular distribution of ectopically expressed hENDOV proteins.

Fig 5

HEK 293T cells were transiently transfected for expression of GFP fused hENDOV isoforms (GFP-hEV 282, GFP-hEV 308, GFP-hEV 309, GFP-hEV 250, GFP-hEV 282AA and GFP-hEV 282-E3) (green). Cells were stained with a Fibrillarin antibody to visualize nucleoli (red). Nuclei are shown by DAPI staining (blue) and colocalization is shown in merged images. Arrows show dots representing nucleolar localization of GFP-hEV isoforms. Confocal images were acquired using an x40 oil objective, with 6–8x zoom (Leica SP8). Bar 5μm.