Table 2. MiRNA levels according to asthma phenotypes.
| Development set | |||||||||
| Asthmaa (n = 40) | Allergic asthma (n = 18) | Eosinophilic asthma (n = 12) | Obese asthma (n = 15) | Persistent asthma (n = 25) | Symptomatic asthma (n = 26) | BD+S+ (n = 7) | BD+S- (n = 14) | BD-S+ (n = 19) | |
| MicroRNA | |||||||||
| miR-21-5p | 1.83 (0.52; 4.20) | 1.61 (0.22; 4.34) | 1.80 (0.56; 3.80) | 1.36 (0.17; 4.06) | 1.75 (0.40; 4.34) | 1.17 (0.40; 3.61) | 1.36 (0.55; 2.71) | 2.85 (1.57; 5.62) | 0.85 (0.22; 4.34) |
| miR-126-3p | 0.11 (1.37x10-4; 0.96) | 2.79x10-3 (1.37x10-4; 0.37) | 7.85x10-3 (1.40x10-4; 0.24) | 0.20 (1.37x10-4; 1.42) | 0.06 (1.37x10-4; 1.42) | 0.04 (1.37x10-4; 0.20) | 1.37x10-4 (1.37x10-4; 0.27) | 0.44 (1.37x10-4; 1.34) | 0.09 (1.37x10-4; 0.20) |
| miR-133a-3p | 0.08 (6.90x10-5; 0.53) | 0.01 (6.85x10-5; 0.42) | 0.02 (7.00x10-5; 0.38) | 0.11 (6.85x10-5; 0.44) | 0.02 (6.85x10-5; 0.44) | 2.24x10-3 (6.85x10-5; 0.33) | 6.85x10-5 (6.85x10-5; 0.32) | 0.26 (0.04; 1.24) | 2.96x10-3 (6.85x10-5; 0.44) |
| miR-145-5p | 0.59 (0.23; 1.32) | 0.54 (0.15; 1.30) | 0.36 (0.11; 0.84) | 0.59 (0.24; 1.30) | 0.59 (0.20; 1.35) | 0.43 (0.15; 1.25) | 0.36 (1.20x10-3; 0.63) | 0.76 (0.49; 2.56) | 0.59 (0.20; 1.35) |
| miR-146a-5p | 6.65x10-4 (6.65x10-4; 0.04) | 6.65x10-4 (6.65x10-4; 0.04) | 6.67x10-4 (6.67x10-4; 0.05) | 6.65x10-4 (6.65x10-4; 0.04) | 4.08x10-3 (6.65x10-4; 0.05) | 6.67x10-4 (6.67x10-4; 0.05) | 6.67x10-4 (6.67x10-4; 0.05) | 6.67x10-4 (6.67x10-4; 0.04) | 6.65x10-4 (6.65x10-4; 0.03) |
| miR-155-5p | 1.19x10-4 (1.19x10-4; 1.19x10-4) | 1.19x10-4 (1.19x10-4; 1.19x10-4) | 1.19x10-4 (1.19x10-4; 1.19x10-4) | 1.19x10-4 (1.19x10-4; 1.19x10-4) | 1.19x10-4 (1.19x10-4; 1.19x10-4) | 1.19x10-4 (1.19x10-4; 1.19x10-4) | 1.19x10-4 (1.19x10-4; 1.19x10-4) | 1.19x10-4 (1.19x10-4; 1.19x10-4) | 1.19x10-4 (1.19x10-4; 1.19x10-4) |
| miR-221-3p | 0.04 (1.89x10-3; 0.29) | 0.03 (1.89x10-3; 0.13) | 0.06 (1.89x10-3; 0.46) | 0.01 (1.89x10-3; 0.33) | 0.05 (1.89x10-3; 0.24) | 0.02 (1.89x10-3; 0.21) | 1.89x10-3 (1.89x10-3; 0.36) | 0.153 (1.89x10-3; 0.83) | 0.03 (1.89x10-3; 0.10) |
| miR-328-3p | 0.44 (0.05; 1.08) | 0.44 (0.08; 0.91) | 0.42 (0.03; 0.90) | 0.24 (5.98x10-3; 1.00) | 0.33 (0.02; 1.00) | 0.29 (5.98x10-3; 0.90) | 0.24 (5.98x10-3; 0.68) | 0.85 (0.27; 1.78) | 0.33 (5.98x10-3; 1.48) |
| miR-423-3p | 1.07x10-3 (1.07x10-3; 1.07x10-3) | 1.07x10-3 (1.07x10-3; 2.15x10-3) | 1.07x10-3 (1.07x10-3; 0.04) | 1.07x10-3 (1.07x10-3; 1.07x10-3) | 1.07x10-3 (1.07x10-3; 1.07x10-3) | 1.07x10-3 (1.07x10-3; 1.07x10-3) | 1.07x10-3 (1.07x10-3; 1.07x10-3) | 1.07x10-3 (1.07x10-3; 1.07x10-3) | 1.07x10-3 (1.07x10-3; 2.15x10-3) |
| Validation set | |||||||||
| Asthmaa (n = 31) | Allergic asthma (n = 14) | Eosinophilic asthma (n = 7) | Obese asthma (n = 11) | Persistent asthma (n = 15) | Symptomatic asthma (n = 17) | BD+S+ (n = 3) | BD+S- (n = 14) | BD-S+ (n = 14) | |
| miR-21-5p | 3.37 (1.35; 5.93) | 3.29 (0.67; 3.91) | 3.22 (1.35; 3.91) | 2.88 (1.54; 3.91) | 3.44 (2.00; 4.04) | 3.22 (2.00; 3.81) | 2.88 (0.25; 10.9) | 3.29 (2.00; 3.81) | 3.29 (2.00; 3.81) |
| miR-126-3p | 0.03 (1.37x10-4; 0.29) | 0.03 (1.37x10-4; 0.24) | 7.85x10-3 (1.37x10-4; 0.24) | 0.15 (7.05x10-3; 1.18) | 0.02 (1.37x10-4; 0.15) | 0.03 (1.37x10-4; 0.15) | 0.05 (2.72x10-4; 0.29) | 0.03 (1.37x10-4; 0.15) | 0.03 (1.37x10-4; 0.15) |
| miR-133a-3p | 0.03 (6.90x10-5; 0.38) | 0.05 (6.85x10-5; 0.38) | 0.03 (6.90x10-5; 0.43) | 0.06 (6.85x10-5; 0.37) | 0.06 (6.85x10-5; 0.38) | 0.03 (6.85x10-5; 0.12) | 8.76x10-3 (6.85x10-5; 0.07) | 0.05 (6.85x10-5; 0.38) | 0.05 (6.85x10-5; 0.38) |
| miR-145-5p | 0.44 (0.17; 1.18) | 0.74 (0.15; 1.60) | 0.17 (9.23x10-3; 1.01) | 0.48 (0.24; 1.18) | 0.49 (0.25; 1.60) | 0.48 (0.19; 1.04) | 0.29 (0.19; 0.54) | 0.49 (0.11; 1.33) | 0.49 (0.11; 1.33) |
| miR-146a-5p | 6.65x10-4 (6.65x10-4; 0.02) | 6.65x10-4 (6.65x10-4; 0.02) | 6.65x10-4 (6.65x10-4; 6.65x10-4) | 6.65x10-4 (6.65x10-4; 6.65x10-4) | 6.65x10-4 (6.65x10-4; 6.65x10-4) | 6.65x10-4 (6.65x10-4; 6.65x10-4) | 0.03 (6.65x10-3; 0.32) | 6.65x10-4 (6.65x10-4; 6.65x10-4) | 6.65x10-4 (6.65x10-4; 6.65x10-4) |
| miR-155-5p | 1.19x10-4 (1.19x10-4; 1.19x10-4) | 1.19x10-4 (1.19x10-4; 1.19x10-4) | 1.19x10-4 (1.19x10-4; 1.19x10-4) | 1.19x10-4 (1.19x10-4; 2.07x10-3) | 1.19x10-4 (1.19x10-4; 1.60x10-3) | 1.19x10-4 (1.19x10-4; 1.19x10-4) | 1.19x10-4 (1.19x10-4; 0.03) | 1.19x10-4 (1.19x10-4; 1.19x10-4) | 1.19x10-4 (1.19x10-4; 1.19x10-4) |
| miR-221-3p | 0.13 (1.89x10-3; 0.44) | 0.12 (1.89x10-3; 0.42) | 0.08 (1.89x10-3; 0.61) | 0.18 (1.89x10-3; 0.69) | 0.18 (1.89x10-3; 0.61) | 0.13 (1.89x10-3; 0.49) | 0.13 (1.89x10-3; 0.46) | 0.15 (1.89x10-3; 0.61) | 0.15 (1.89x10-3; 0.61) |
| miR-328-3p | 0.55 (5.98x10-4; 1.37) | 1.04 (0.55; 2.32)** | 0.61 (0.03; 1.61) | 0.26 (0.08; 0.98) | 0.89 (0.42; 2.32)* | 0.63 (0.42; 1.37) | 0.25 (5.98x10-4; 0.63) | 0.94 (0.47; 1.61) | 0.94 (0.47; 1.61)* |
| miR-423-3p | 1.07x10-3 (1.07x10-3; 0.10) | 1.07x10-3 (1.07x10-3; 0.05) | 1.07x10-3 (1.07x10-3; 0.09) | 1.07x10-3 (1.07x10-3; 0.19) | 1.07x10-3 (1.07x10-3; 0.10) | 1.07x10-3 (1.07x10-3; 0.10) | 0.19 (1.07x10-3; 0.22) | 1.07x10-3 (1.07x10-3; 0.07) | 1.07x10-3 (1.07x10-3; 0.07) |
Data are expressed as medians (25th-75th percentile)
a: asthma was defined based on positive bronchodilation or self-reported medical diagnosis with reported symptoms in the previous year
Allergic asthma: defined by “asthma” in a child with positive skin prick test
Eosinophilic asthma: defined by “asthma” in a child with exhaled nitric oxide above 35 ppb
Obese asthma: defined by “asthma” in an overweight or obese child
Persistent asthma: defined by “asthma” in a child currently using anti-asthma medication
Symptomatic asthma: defined by “asthma” in a child with current symptoms
BD+S+: Positive bronchodilation with asthma symptoms defined by “asthma” in a child with a current positive bronchodilation test and symptoms
BD+S-: Positive bronchodilation without asthma symptoms defined by “asthma” in a child with a current positive bronchodilation test and without symptoms
BD-S+: Negative bronchodilation with asthma symptoms defined by “asthma” in a child with a current negative bronchodilation test and with symptoms
Significant differences in bold
*: p-value <0.05
**: p-value <0.01