Figure 5. SCGN loss leads to increased sensitivity to DSS colitis.

(a–c) Body weight, disease activity index (DAI), and survival of male WT (n = 13) and Secret1 (n = 13) mice treated for 6 days with 2% DSS in their drinking water. (d) Histologic score of male and female WT (n = 11) and Secret1 (n = 13) animals treated for 6 days with 2% DSS. (e) Representative microphotographs (20x) of colonic epithelium of DSS-treated mice. (f) Inflammatory gene expression measured by qRT-PCR from ceca of DSS-treated mice. Data in (a), (b) and (c) are representative of 2 experiments. *p<0.05. **p<0.01, two tailed unpaired t test in (a), (b) and (f). *p<0.05. one tailed unpaired t test in (d). S.E.M. was used for error bars in (a), (b), (d), (f). Log rank test was used in (c).

Figure 5—figure supplement 1. Increased sensitivity to DSS in Scgn deficient animals.

(a) Body weight of male and female Secret1 (n = 27) and WT (n = 21) mice treated with 2% DSS for 6 days. This is the aggregate data of 2 separate experiments. (b) Body weight of male Secret2 and WT mice during 6 day treatment with 1% DSS. (c) Inflammatory gene expression from ceca of experimental mice as measured by qRT-PCR. WT n = 9 Secret2 n = 12. **p<0.01 two tailed unpaired t test in (b) *p<0.05 one tailed unpaired t test in (c). Error bars in (a), (b), (c). S.E.M.

Figure 5—figure supplement 2. Immunophenotying of WT and Secret1 mice.

Lamina propria leukocytes were extracted from colon and small intestine (SI) of WT and Secret1 mice and stained for flow cytometry. The flow cytometry images show pooled analysis of (a) CD45⁺CD4⁺ cells T cells, (b) CD45⁺CD19⁺ B cells, (c) CD45⁺NK1.1⁺ NKT cells, (d) CD45⁺CD4⁺CD25⁺ Tregs, (e) CD45⁺Lin^–NKp46^–IL23R⁺ ILC3, (f) CD45⁺CD11b⁺Ly6G⁺ neutrophils, (g) CD45⁺CD11b⁺ macrophages, and (h) CD45⁺CD11b⁺CD11c⁺ dendritic cells. (i) Representative images showing gross appearance of the spleen and MLN from WT and Secret1 mice (n = 5 mice in each group).