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. 2019 Sep;61(3):322–331. doi: 10.1165/rcmb.2018-0326OC

Figure 1.

Figure 1.

SMAD signaling activity is suppressed in differentiated goblet cells in human airway epithelium. (A and B) Costaining of phosphorylated (p)-SMAD1/5/8 (A) and p-SMAD2/3 (B) with goblet cell marker (MUC5AC), ciliated cell marker (FOXJ1), and basal cell marker (p63) in human bronchial epithelium. The nucleus is stained by DAPI. Scale bars: 10 μm. Insets show a fourfold-enlarged portion of the staining image to highlight finer detail. (C) Costaining of p-SMAD1/5/8 and p-SMAD2/3 (mixed antibodies) with goblet cell marker (MUC5AC), club cell marker (CCSP), ciliated cell marker (FOXJ1), and basal cell marker (CK5) in human airway epithelium differentiated on air–liquid interface (ALI). The nucleus is stained by DAPI. Scale bars: 10 μm. The dotted lines outline the shape of club cells or goblet cells. The staining shown was performed from bronchial sections of one representative donor. Staining has been replicated in n = 4 donors and similar results were observed.