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. 2019 Nov 8;10:5093. doi: 10.1038/s41467-019-13074-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Auxin output minimum correlates with apical stem cells. a Confocal readout from R2D2 auxin input sensor. b Ratiometric representation of R2D2 activity in the epidermal cell layer (L1). c Quantification of averaged pDR5v2:ER-eYFP-HDEL and pCLV3:mCherry-NLS distribution (n = 5). d Confocal readout from pDR5v2:ER-eYFP-HDEL auxin output reporter. e pCLV3:mCherry-NLS stem cell marker in the same SAM. f Computational subtraction of L1 signals shown in d and e. Relative signal intensity is shown in arbitrary units. Scale bars: 50 µm