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. 2019 Nov 8;10:5093. doi: 10.1038/s41467-019-13074-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Stem cells require auxin signaling to remain active, but are resistant to overactivation of the pathway. a pDR5v2:ER-eYFP-HDEL activity in plants harboring pCLV3:BDL-D-GR after 24 h of mock treatment. b DR5v2 signal after 24 h of DEX treatment. c, d Representative phenotypes of lines expressing pCLV3:BDL-D (c) and pCLV3:BDL (d). e–l Response of DR5v2 and CLV3 to induction of pCLV3:BDL-D-GR over time. pDR5v2:ER-eYFP-HDEL shown in green, pCLV3:mCherry-NLS shown in magenta. CLV3 signal is expanded and sustained until the meristem terminates. i–l Mock treated pCLV3:BDL-D-GR controls. m–o Activity of DR5v2 in SAMs with enhanced auxin signaling in the central zone. m wild type control, (n) pCLV3:MP, (o) pCLV3:MP∆. p SAM size quantifications for plants carrying pCLV3:GFP, pCLV3:MP, or pCLV3:MP∆ in two independent T1 populations. q Quantification of pCLV3:mCherry-NLS signal strength in wild type (n = 4). r Quantification of pDR5v2:ER-eYFP-HDEL signal strength in wild type (red, n = 4), pCLV3:MP (light blue, n = 4) and pCLV3:MP∆ (purple, n = 4). In q and r kernel regression was used to visualize dependence of fluorescence signal from SAM center. 95% confidence intervals were simulated using bootstrapping (10.000 iterations) and are shown in lighter colors. s, t Representative phenotypes of lines expressing pCLV3:MP∆ (s) and pHMG:MP∆ (t). All scale bars 50 µm, except c and d 2 mm; (s) and (t) 3.5 mm